Fig. 4
From: Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

Pollen tubes of Phalaenopsis Little Gem Stripes grown in stigma and ovary cavity of P. Sogo Vivien ‘F858’ after storage. a–d The pollen taken from storage for 96 weeks at four temperatures was pollinated for 7 days. a room temperature storage; b 4 °C; c − 20 °C; d − 80 °C. e Pollen from storage at − 20 °C was pollinated for 35 days, and f for 70 days, g partially magnified from e. Arrow indicates pollen tube (c, d, and f). P, pollinia; O, ovules. Scale bars = 50 μm