Fig. 2From: Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum)GUS staining results of ice plant seedlings infected with the A. tumefaciens EHA105 strain harboring the pBISN1 binary vector. Representative GUS staining results of 5- (a–d), 3- (e, f), and 7-day-old (g, h) seedlings. Blue circles indicate blue spots in intact seedlings (a, e, g); seedlings with root-tip-removed (b, f, h); shoot (c) and root (d). Tissues showing blue spots indicate successful transformation. Bar = 2 mmBack to article page