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Fig. 3 | Botanical Studies

Fig. 3

From: Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum)

Fig. 3

GUS staining results and genomic DNA PCR results of ice plant seedlings infected with A. rhizogenes A4 or A8196 strain harboring the pCAMBIA1303 binary vector. a, b Representative GUS staining results of 3- and 5-day-old seedlings infected with A. rhizogenes A4 or A8196 at 2 days after infection and uninfected (control) seedlings. Bar = 5 mm. c, d Genomic DNA was isolated from cotyledons (C) and roots (R) of 3- and 5-day-old infected and uninfected (control) seedlings. Distilled water was a negative control. The pCAMBIA1303 (1303) plasmid was used as a positive control for the PCR amplifications with the GUS reporter gene and the kanamycin resistance gene (Kan). Primers PPC1 3′–5 and PPC1 3′–3 were used to amplify the endogenous PPC1 gene; primers GUS1 and GUS2 were used to amplify GUS reporter gene (GUS); primers Kan-F and Kan-R were used to amplify kanamycin resistance gene (Kan)

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