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Table 1 Transient transformation rates of A. tumefaciens EHA105 strain harboring the pBISN1 plasmid in ice plant seedlings and distribution (%) of GUS staining in ice plant seedlings of different ages

From: Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum)

AgesPlant typesTransient transformation ratesa% of tissues showing GUS activitiesb
3-day-oldIntact seedling66.7 ± 2.8*Cotyledon100.0 ± 2.8#
Hypocotyl14.3 ± 0.4
Root tip removed85.0 ± 1.1*Cotyledon88.9 ± 0.9#
Hypocotyl22.2 ± 0.2
5-day-oldIntact seedling48.3 ± 3.8*Cotyledon63.4 ± 1.3#
Hypocotyl52.1 ± 2.5
Root tip removed80.0 ± 2.0*Cotyledon98.2 ± 2.0#
Hypocotyl25.7 ± 0.3
7-day-oldIntact seedling0.0 ± 0.00.0 ± 0.0
Root tip removed13.3 ± 2.8Cotyledon0.0 ± 0.0#
Hypocotyl100.0 ± 2.8
  1. aNumber of transiently transformed seedlings/total number of seedlings × 100%. The 60–80 ice plant seedlings were infected with A. tumefaciens strain for each independent transformation assay. Data are mean ± SD (standard deviation) from at least three independent experiments
  2. bNumber of GUS stained tissues/number of transiently transformed seedlings × 100%
  3. * P < 0.05 comparing 3-, or 5-day-old infected seedlings and 7-day-old infected seedlings of the same ice plant type based on pairwise Student t-test
  4. #P < 0.05 comparing cotyledon and hypocotyl tissues of the same age and type ice plants based on pairwise Student t-test
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