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Table 2 Transient transformation rates of A. rhizogenes A4 or A8196 strain harboring the pCAMBIA1303 plasmid in ice plant seedlings and distributions (%) of GUS staining in different tissues of 3- and 5-day-old ice plant seedlings

From: Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum)

Ages of seedlingsA. rhizogenes strainsDays after 2-day coincubation periodsTransient transformation ratesa% of tissues showing GUS activitiesb
3-day-old with root tip removedA41100.0 ± 0.0*Cotyledon100.0 ± 0.0#
Hypocotyl0.0 ± 0.0
Root95.0 ± 0.9#
A81961100.0 ± 0.0*Cotyledon100.0 ± 0.0#
Hypocotyl0.0 ± 0.0
Root100.0 ± 0.0#
A4296.7 ± 1.1*Cotyledon96.7 ± 0.7#
Hypocotyl6.3 ± 0.1
Root95.3 ± 0.1#
A81962100.0 ± 0.0*Cotyledon100.0 ± 0.0#
Hypocotyl5.6 ± 0.0
Root100.0 ± 0.0#
A4592.9 ± 2.3*Cotyledon83.3 ± 0.5#
Hypocotyl1.9 ± 0.6
Root85.2 ± 0.5#
A81965100.0 ± 0.0*Cotyledon100.0 ± 0.0#
Hypocotyl0.0 ± 0.0
Root100.0 ± 0.0#
A4733.3 ± 1.1Cotyledon33.3 ± 1.1#
Hypocotyl0.0 ± 0.0
Root31.4 ± 1.0#
A819670.0 ± 0.0Cotyledon0.0 ± 0.0
Hypocotyl0.0 ± 0.0
Root0.0 ± 0.0
5-day-old with root tip removedA41100.0 ± 0.0*Cotyledon66.7 ± 1.1#
Hypocotyl0.0 ± 0.0
Root55.0 ± 0.9#
A81961100.0 ± 0.0*Cotyledon45.0 ± 1.2#
Hypocotyl0.0 ± 0.0
Root47.5 ± 1.2#
A4297.5 ± 0.6*Cotyledon65.0 ± 1.0#
Hypocotyl0.0 ± 0.0
Root55.0 ± 0.9#
A81962100.0 ± 0.0*Cotyledon50.0 ± 1.3#
Hypocotyl2.4 ± 0.1
Root47.6 ± 1.2#
A4547.2 ± 1.2Cotyledon31.5 ± 1.0#
Hypocotyl0.0 ± 0.0
Root29.6 ± 0.9#
A819650.0 ± 0.0Cotyledon0.0 ± 0.0
Hypocotyl0.0 ± 0.0
Root0.0 ± 0.0
A4745.0 ± 0.0Cotyledon28.3 ± 0.9#
Hypocotyl0.0 ± 0.0
Root30.0 ± 0.9#
A819670.0 ± 0.0Cotyledon0.0 ± 0.0
Hypocotyl0.0 ± 0.0
Root0.0 ± 0.0
  1. aNumber of transiently transformed seedlings/total number of seedlings × 100%. The 60 to 80 ice plant seedlings were infected with A. rhizogenes strain for each independent transformation assay. Data are mean ± SD (standard deviation) from at least 3 independent experiments
  2. bNumber of GUS stained tissues/number of transiently transformed seedlings × 100%
  3. * P < 0.05 comparing 1-, 2-, or 5-day after infection and 7-day after infection of the same ice plant type under the same bacteria strain infection condition based on pairwise Student t-test
  4. #P < 0.05 comparing cotyledon, or root tissues and hypocotyl tissues of the same type ice plants under the same bacteria infection condition based on pairwise Student t-test
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