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Botanical Studies
Fig. 2 | Botanical Studies

Fig. 2

From: Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene

Fig. 2

Molecular characterization of GA2ox6-OX transgenic lines. a Genomic DNA PCR. Primers specific to the T-DNA vector construct (Ubi-F/GA-R) and the Hpt gene (Hpt-F/Hpt-R) were used to verify the integration of the transgene. Four GA2ox6-OX transgenic lines, (#1-1, #3-2, #3-3 and #3-4), were analyzed. NT: nontransgenic line. b GUS activity assay. Leaf and root samples from the NT line and four GA2ox6-OX lines were analyzed. c RT-PCR. The OsGA2ox6 transgene expression in the NT line and four GA2ox6-OX lines was analyzed by RT-PCR, and the expression of the actin gene (from Phalaenopsis) was used as an internal control

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