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Botanical Studies

Table 1 Potato brown rot incidence under different algal treatments conditions

From: Potential of algal-based products for the management of potato brown rot disease

 

Location 1

 

Location 2

 

Treatment

Soil

 

Tubers

Soil

 

Tubers

 
 

(CFU/g)3

SMSA

IFAS4

SMSA

IFAS

RT-PCR5

(CFU/g)

SMSA

IFAS

SMSA

IFAS

RT-PCR

 

Untreated soil (control)

1.82 ± 1.22

+ve

2.22 ± 1.52

+ve

23.4

3.07 ± 2.83

+ve

2.07 ± 1.82

+ve

34.4

 

A. spicifera1

2.00 ± 1.46

+ve

2.00 ± 1.76

+ve

26.72

2.12 ± 1.95

+ve

0.00 ± 0.00

 

Und.

 

S. platensis1

1.92 ± 1.64

+ve

0.00 ± 0.00

 

Und**.

2.37 ± 2.12

+ve

2.48 ± 1.76

+ve

22.03

 

T. ornate2

0.00 ± 0.00

 

0.00 ± 0.00

 

44.05*

0.00 ± 0.00

 

0.00 ± 0.00

 

Und.

 

C. racemose : C. myrica

0.00 ± 0.00

 

0.00 ± 0.00

 

Und.

0.00 ± 0.00

 

0.00 ± 0.00

 

Und.

 

(mix 1:1)2

           
  1. 1 Algal extract 2 dried algal biomass (DAB) 3Log10 (Mean ± SE)
  2. 4IFAS ((Antiserum (anti-goat) and Rabbit anti-Goat antibody conjugated with FITC): a confirmatory test for typical colonies developed on SMSA
  3. 5Real-time PCR on R. solanacearum (RS-I-F, RS-II-R) and the fluorescent signal generated by RS-P (FAM) (Weller et al. 2000) using the Biorad cfx96 machine, real-time PCR.
  4. PC is R. solanacearum race 3 biovar 2 Phylotype II, sequevar 1 culture, previously isolated and identified (Hamed and Messiha 2018)
  5. *CT > 40 is considered negative, ** undetermined CT values
  6. Location 3: The pathogen was undetectable in all treatments.
  7. SMSA: South Africa Selective Media (Anonymous 1998). IFAS: Immunofluorescence Antibody Staining. (Janse 1988).
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