Studies of life history of Gagea graeca (Liliaceae) based on morphological and molecular methods
© The Author(s) 2017
Received: 29 December 2016
Accepted: 25 September 2017
Published: 3 October 2017
We studied the life history of Gagea graeca (L.) A. Terracc. (sect. Anthericoides) by field surveys on the Greek island of Crete, including quantitative analyses of 405 individuals, estimation of resource allocation by measuring the nitrogen content of different plant organs, assessing seed set and recording genetic diversity via amplified fragment length polymorphism (AFLP) analyses. In contrast to most species of the genus G. graeca seems to be a short-lived perennial, developing several characters that are rather typical for annual plants.
Although seed set varies largely, flowering plants produce many (68 ± 79) small, flattened seeds (mean weight 73 ± 22 µg) in comparison to a single bulbil. If measured as nitrogen content of the respective plant parts, investment in seeds (25%) is much higher than that in bulbils (4%). In addition, the threshold for flower formation (expressed as bulb size where 50% of the plants form the respective structure) is with 2.17 ± 0.05 mm lower than that for bulbils with 2.80 ± 0.16 mm. This is in accordance with AFLP analyses revealing predominantly sexual reproduction (only 9.1% of 110 investigated plants belonged to clones).
In the genus Gagea early, predominantly sexual reproduction seems to be characteristic for species from arid habitats, coupled with a low proportion of clonal plants.
Gagea graeca (L.) A. Terracc. is one of the few white-flowering species in the genus Gagea Salisb. (Liliaceae). Together with the closely related G. trinervia (Viv.) Greuter (Peruzzi et al. 2008) it is assigned to section Anthericoides A. Terracc. This monophyletic section (e.g., Peruzzi et al. 2008; Peterson et al. 2008, 2011, 2016; Zarrei et al. 2009) within the genus Gagea (incl. Lloydia Rchb.; see Peterson et al. 2004, 2008; Peruzzi et al. 2008; Zarrei et al. 2011) is currently accepted in all infra-generic classifications (Levichev in Peterson et al. 2008; Zarrei et al. 2011; Peruzzi 2012a). Both species are endemic to the Mediterranean region and are diploid (2n = 24; Peruzzi 2003, 2008, 2012a). In several phylogenetic studies (e.g., Peruzzi et al. 2008; Peterson et al. 2011) the section Anthericoides was found to be in a sister position to other studied sections of the genus.
Plants of G. graeca germinate with a single leaf, but in subsequent years the number of basal leaves grows to 2, (sometimes to 3 or 4; Levichev in Peterson et al. 2008). According to Peruzzi et al. (2008) plants can produce a first flower already in their second year. In adult plants usually several flowers were observed. Compared with the size of the plant, flowers are showy, with white, elongated tepals with reddish veins, which are recurved at apex. This habit, resembling that of an annual plant, is unusual among the genus and points to prevailing reproduction via seeds, whereas most other species of the genus reproduce predominantly via bulbils (e.g., Levichev 1999, 2013; Schnittler et al. 2013). However, in addition to the renewal bulb produced in the axil of the first leaf (obligatory for the genus, furthermore named parent bulb) plants with two leaves start to produce a first bulbil on a very short stolon (hypopodium according to Peruzzi et al. 2008). As such, vegetative reproduction is regularly available as a second option. The other species of section Anthericoides, Gagea trinervia differs from G. graeca in “having bulbils with stolon-like hypopodium, a regular sister bulbil at the immature stage, an unifacial, juvenile-like basal leaf to adult stage, few cauline leaves, few flowers and a low level of sexual reproduction” (Peruzzi et al. 2008).
Gagea graeca can be found in various, usually slightly disturbed, open habitats like dry grasslands, rocky slopes and fallow fields especially on soils that are provided with some moisture to prevent the relatively small bulb from desiccation over the summer. In Crete, where the species was investigated, most of the ancient woodlands are now replaced by low, thorny shrubs (a vegetation called “phrygana”) and open grasslands (Zimowski et al. 2014). Here G. graeca usually inhabits small gullies in where the vegetation cover does not exceed 50%. At such places, the plant can form large populations comprising thousands of individuals.
This investigation was undertaken to study adaptations of Gagea graeca on its Mediterranean environment, and compare these adaptations with those of another species of the genus adapted to arid environments, G. bulbifera (Pall). Salisb. (Beisenova et al. 2015). A quantitative morphology approach (Schnittler et al. 2009, 2013; Beisenova et al. 2015), including measurements on living plants of all ontogenetic stages, is used to quantify possible traits enabling G. graeca to persist in the only temporarily wet vegetation Mediterranean shrub land. In addition, amplified fragment length polymorphism (AFLP), (Vos et al. 1995) was employed to estimate the proportions of vegetative (leading to clones) and generative reproduction (producing new genotypes) in the species.
Numbers of plants investigated for morphological measurements, C/N data, seed production and seed weight, and AFLP analyses from the four investigated populations
I. Central Crete, Psilioritis Mts., Anogia, along a little brook in phrygana ca. 1 km SE Gonies, road fom Gonies to Anogia, at a branch that leads to a windmill, slopes with fine soil between rocks (flysch), elev. 600 ± 25 m a.s.l., 35°17′58 “N, 24°55′36″ E ± 25 m,
II. Central Crete, southern coast near Plakias, temporarily wet fallow field ca. 800 m SE Kanevos village, along the gravel road to a small chapel, ascent to Kouroupu hills, elev. 476 ± 25 m a.s.l., 35°13 ‘37″ N, 24°24′10″ E ± 25 m,
III. Western Crete, Levka Ori Mts., Chania, rocks and fallow Olive orchards in Quercus ilex/Pistacia lentiscus woodlands: about 1 km N Tsakistra village, road between Tsakistra and Kampoi, elev. 581 ± 30 m a.s.l., 35°24′33″ N, 24°04′16″ E ± 25 m,
IV. Central Crete, south of Knossos/Spilia near Iraklio, phrygana in succession to Quercus shrubland: ca. 1450 m SW of Kera Eleousa village, elev. 222 ± 20 m a.s.l., 35°15′52″ N, 25°09′38″ E ± 25 m.
All morphological studies were carried out on living plants towards the end of the flowering season (March 15–30, 2014) from populations I and II (Table 1). At this time, most of the plants already developed fruits (capsules); a few plants were still in bloom. Of these 405 plants, 114 were in non-flowering condition.
Using a ruler and/or a digital caliper (precision ± 0.02 mm) we measured the diameter of the replacement bulbs (including the thin tunic of the former parent bulb; see Schnittler et al. 2013) and the diameter/length of the usually single basal bulbil and its short to extremely short stolon. In addition, we recorded number of flowers, shoot height above ground, and numbers of basal and stem leaves. For all measures, the standard deviation (SD) is given. To determine a threshold for the diameter of the replacement bulb needed to develop bulbils and/or flowers, these graphs were fitted against the equation y = 1/[1 + e−(x−xo)/b] which describes a sigmoid function with xo as the threshold (the turning point of its slope). For the graphs, flowers with only the male function (aborted and much smaller capsules) were counted as 0.5, perfect flowers as 1 (capsules are of normal size and fertile).
About a month later (between May 1 and May 20), the number of seeds per plant was counted for 177 plants from populations I, II and IV (Table 1). To record variation in seed production, all formerly flowering plants (those with a developed shoot) for a certain area (1–2 m2) were collected. At this time, capsules were fully developed and all vegetative plant parts were dried up. In a few plants capsules started to dehisce; such plants were not collected.
Material from populations I, II and IV (Table 1) was used to determine absolute contents of carbon and nitrogen. Plants were dried for about 4 days in phosphorus pentoxide, and dry mass of the respective plant parts (seeds, replacement bulbs and vegetative structures) was determined with a precision of ± 1 µg (for seeds, 100 seeds were pooled for weighing). Several items of these plant structures were cut or pooled in a way that dry mass ranges between 6 and 15 mg. This weight corresponds with one average-sized replacement bulb, vegetative remains from a single plant, or ca. 100 seeds. Using replicate samples, a total of five replicates were measured except for basal bulbils. This procedure yielded 42 samples in total which were wrapped into tin foil and analyzed for carbon (C) and nitrogen (N) content with an automatic analyzer Vario EL III. Using the means of 3–10 samples, N-contents were calculated and used as a resource estimator (compare Ashman 1994). Figures for N content per mg dry mass were used to estimate nitrogen content of the respective plant parts, and calculate their relative investments in terms of nitrogen incorporated.
For a total of 110 sampled plants from populations I and II (Table 1) AFLP profiles were generated, using the methods described in Pfeiffer et al. (2011). For these analyses two transects (populations I and II with 27 and 28 pairs of plants, respectively, see Table 1) were sampled. We employed the sampling scheme of Pfeiffer et al. (2011), where pairs of plants (maximum distance 10 cm) were collected along a transect line (minimum distance between pairs 1 m).
We did five/six full replicates (including DNA extraction) for the first/second transect to determine the error rate. Due to the high DNA content of many Liliaceae (Veselý et al. 2012; Zonneveld et al. 2015), we used a primer combination (EcoRI-AGCG plus Vsp-GCAT) with four selective bases. The first selective base was added to the pre-amplification primers, and all four were added to the primers used in the main amplification. The electroferograms obtained with a sequencer ABI 310 (applied biosystems) were binned and the alleles, defined as peak presence/absence, were read automatically to a 0/1 matrix using GenMapper 4.0 software (applied biosystems). To identify clones, an algorithm was programmed in Microsoft Excel which does not only check for identity, but allows to define a threshold to account for genotyping (biological, experimental, and scoring) errors (Douhovnikoff and Dodd 2003; Schnittler and Eusemann 2010). This threshold was derived from histograms of pairwise distances between samples. The histograms showed a bimodal distribution with two peaks, one for non-identical plants, and one for putative clones, with a local minimum (the threshold) in between. This threshold was below the pairwise distances between replicates. All samples with AFLP profiles deviating from each other in more alleles than defined by the threshold were regarded as independent genotypes.
Life history characteristics of two species of Gagea from arid environments (Gagea graeca, G. bulbifera) compared with a species from temperate deciduous woodlands (G. lutea)
Beisenova et al. (2015)
Bulb size, flowering plants
3.01 ± 0.63
2.55 ± 0.48
8.94 ± 1.62
Threshold (mm bulb diam.) for
2.17 ± 0.05
1.90 ± 0.13
6.94 ± 0.04
2.80 ± 0.16
4.96 ± 11.39
2.63 ± 0.21
Bulbils per plant showing bulbils (range)
1.01 ± 0.10 (1–2)
1.00 ± 0.00
8.65 ± 5.31 (1–23)
Average bulbil size
1.26 ± 0.39
1.11 ± 0.48
1.49 ± 0.42
Flowers per plant
1.9 ± 1.1 (1–7)
1.4 ± 0.9 (1–9)
3.2 ± 1.5 (1–8)
Capsules per fruiting plant
1.2 ± 0.6 (1–4)
1.4 ± 0.9 (0–9)
3.2 ± 1.5 (0–8)
Mean seed weight (µg)
73 ± 22
80 ± 20
1650 ± 340
Seeds per flowering plant
68 ± 79 (0–445)
119 ± 90 (0–827)
41 ± 30 (0–159)
Seeds per fruiting plant
98 ± 77 (0–445)
130 ± 86 (0–827)
41 ± 30 (0–159)
Seeds per capsule
80 ± 39 (0–184)
99 ± 40 (0–224)
12 ± 6 (0–33)
N-content (µg/mg dry weight)
27.9 ± 2.6
23.9 ± 1.8
8.3 ± 3.5
41.2 ± 6.6
22.1 ± 1.8
25.7 ± 1.9
N investment (%) into
Diversity R = (G−1)/(N−1)
Proportion of clonal plants
The replacement bulbs are always close (1–3 cm) to the surface, and are hidden by the reddish-brown sheaths of the basal leaves. The first basal leaf, which is as well the first to decay during flowering season, develops the replacement bulb; the second, very rarely the third as well, develops usually one basal bulbil which is on a saccate enlargement of the basal leaf, which forms a short stolon. Larger non-flowering plants (34 of 114, mean bulb diam. 2.20 ± 0.40) developed a bulbil (Fig. 3d–f). From 291 flowering plants measured, the weaker individuals (131 plants, mean bulb diam. 2.72 ± 0.56) did not yet develop a bulbil, the larger ones (160 plants, mean bulb diam. 3.24 ± 0.69 mm) supported a basal bulbil (diam. 1.26 ± 0.39 mm). Among these were two with a second, smaller basal bulbil (sister bulbil; diam. 1.17 ± 0.36 mm). Bulbils developed often on a short (0.42 ± 0.71 mm, range 0–4.01 mm) stolon. Compared with other species in the genus, the replacement bulbs of G. graeca stay small even in richly flowering plants; the maximum bulb size recorded by us was 5.12 mm.
A total of 177 plants from three populations (Table 1) with altogether 339 flowers and 165 seed capsules were counted. Seed production increased strongly with bulbil size class (Fig. 4c). Plants had on average 1.9 ± 1.1 (1–7) flowers, but the single flower of weak plants and the terminal flower(s) of larger plants did often fail to set seeds. Therefore, only 123 of 177 investigated plants were fertile; this cohort developed 1.2 ± 0.6 capsules with 98 ± 77 (0–445) seeds.
Mean N and C contents measured for bulbs, seeds and vegetative parts of Gagea graeca
27.9 ± 2.6
641.5 ± 62.5
41.2 ± 6.6
486.0 ± 72.6
5.6 ± 0.8
510.1 ± 62.6
With known amounts of plant structures used for measurements, the relative investment of a plant into the respective structures can be estimated. The N content of an average-sized replacement bulb was measured as 470.40 ± 20.05 µg (n = 9). Bulbils were too small and occurred too rarely to measure N content. If the size of a bulb is approximated by the volume of a sphere with the diameter of the respective structure (replacement bulbs: 3.24 ± 0.69 mm; basal bulbils 1.26 ± 0.39 mm), the ratio between average volumes of replacement bulbs and bulbils is 17:1. If we assume that bulbils have the same N-content per unit volume as replacement bulbs, the N content of an average bulbil can be estimated as 27.67 µg. The absolute N content for 100 seeds was measured as 259.97 ± 66.53 µg. Since a plant forms on average 68 ± 79 seeds (including plants with lacking seed set, see Table 2), the N content of 68 seeds would be 176.78 µg. Similarly, the vegetative parts of nine plants were measured (N content 79.10 ± 4.56 µg). Comparison of the absolute N contents of these structures gives the following picture: flowering plants of G. graeca allocate most of their resources (67.1%) to the replacement bulbs, 3.9% to the usually single basal bulbil, 25.2% to an average of 68 seeds, and 3.8% remain in vegetative plant structures.
Gagea graeca differs from other species of Gagea in the Mediterranean area (which are mostly members of the section Didymobulbos, Tison et al. 2013) by its slender habit and the solitary growth of plants. Typically, in species of Gagea relying on reproduction by subterranean bulbils the plants form clusters, whereas plants of G. graeca usually grow apart from each other. A high proportion of the plants in all populations collected by us were always seen in flowering condition. Therefore, sampling for the morphological measurements focused on small, non-flowering plants, to have all classes of parent bulbil size more evenly represented than it would be the case in a random sample from the population. We nevertheless could only achieve a proportion of 28% non-flowering plants. Although G. graeca is certainly a short-lived perennial indicated by the significant resource allocation to replacement bulbs (and true annuals are not known for Liliaceae, Peruzzi 2016) the habit of the plant with its comparatively large flowers resembles that of an annual. In addition, in agreement with Greuter (1970) in our quantitative study we rarely observed sister bulbils in G. graeca (two of 194 plants were seen with two bulbils). Peruzzi et al. (2008) did not found such a structure at any stage of the ontogeny (herbarium collections, cultivated plants).
Measuring or counting a number of morphological traits as demonstrated in Schnittler et al. (2009) quantifies these visible adaptations and allows to relate them to the resource status of the plant, here estimated by the size of the replacement bulb, which carries the plant through the summer dormancy period into the next year (Levichev 1999, 2013). These measurements confirm reports about plant ontogenesis (Peruzzi et al. 2008). Although the age of the plants cannot be determined, the measurements allow to determine threshold (in terms of bulbil size) for the development of seeds and bulbils. For the species of the genus Gagea, the relation between sexual (seeds) and asexual (bulbils) reproduction seems to be a crucial adaptive trait (Schnittler et al. 2013) and is determined by these thresholds (as the diameter of the replacement bulb where 50% of all plants develop the respective structure, Schnittler et al. 2009) and the number of seeds and bulbils. Therefore, resource allocation into seeds and bulbils can be assumed to be under high selective pressure. Several traits should influence the seed/bulbil ratio. First, seeds show a higher desiccation tolerance than bulbils, making them true dormant stages. A second factor, often seen in the genus Gagea, is partial or full sterility caused by high (and especially odd) ploidy levels. This constrains a higher investment into bulbils as the only remaining mode of reproduction. Third, large genome size, as often found in monocotyledonous plants, especially Liliales, (Leitch et al. 2010) tends to complicate meiosis and thus sexual reproduction. Taking these factors into account, species of Gagea in arid habitats should be diploid (or add least have a low even ploidy level) and have small genomes to allow predominant reproduction via seeds as the more drought resistant diaspore type. Indeed, a meta analysis of Liliaceae indicated counter selection against large genomes in arid climates (Carta and Peruzzi 2016).
If the environment allows, increased bulbil production can function as an exit strategy for missing seed set, as shown by a comparison between G. lutea (hexaploid) and G. spathacea (nonaploid, Schnittler et al. 2009): the latter species compensates missing seed set, most likely caused by incorrect pairing of chromosomes due to their odd number, with an increase in the number of basal bulbils and seems to be fully clonal (Pfeiffer et al. 2011). To compare the amount of resources invested into both types of diaspores, we used nitrogen content as a proxy (compare Ashman 1994). Shifts in the bulbil/seed ratio have a strong impact on clonal diversity of a species (since not only numbers, but also establishment probabilities of the respective diaspore types matter), but are as well recognizable by different resource allocation and changes in numbers of these propagules.
Table 2 shows a comparison of the life history traits of a species from humid environments (G. lutea, Schnittler et al. 2009) and two species from arid environments, (Gagea graeca, this study, G. bulbifera, Beisenova et al. 2015). Gagea lutea is a typical species of the species-rich sect. Gagea and inhabits deciduous woodlands and meadows of the humid temperate zone. Gagea graeca (sect. Antericoides) and G. bulbifera (sect. Bulbiferae) occur in arid habitats (Mediterranean shrub lands and Middle/Central Asian steppes, respectively). The latter two species assume different positions in phylogenies of the genus (Peterson et al. 2008), but share nevertheless several key traits that are different from G. lutea. First, both species from arid areas have high fecundity (as indicated by the number of seeds per produced per capsule, Table 2). Second, the threshold for flower formation is lower than for the formation of bulbils. Third, only a single basal bulbil is formed. Fourth, an ontogenetic switch between bulbil and seed production is absent: bulbils and seeds can be produced simultaneously (see discussion below). Fifth, the genetic diversity, calculated as the proportion of singleton genotypes, is rather high due to dominating reproduction via seeds.
Another difference between species from humid and arid regions regards seed morphology: Together with the species of the section Bulbiferae, the two species of sect. Anthericoides share one important character (which may be ancestral for the genus, since it is occurring as well in other Liliaceae genera, Peruzzi 2016): the platyspermous (flattened) seeds. Several other sections (Platyspermum, Plecostigma, Stipitatae) of the genus Gagea are platyspermous, and these assume different positions in phylogenetic trees (Peterson et al. 2008). Many of these species are adapted to arid conditions and inhabit steppes of the Irano-Turanian and Saharo-Arab regions. One possible explanation would be to assume an adaptation to wind dispersal of seeds (Peterson et al. 2008). The comparison with G. lutea (Table 2), a species from humid environments with terete seeds, shows that the low seed weight seems to allow platyspermous species to produce more seeds. This should be an important precondition for an ephemerous life style. Seeds of the humid-zone species are about 20 times heavier than these of the two arid-zone species. Not surprisingly, Gagea lutea produced on average 41 seeds per flowering plant, the average for the studied populations of G. graeca is 68 (but it should be noted that ca. one-third of the sampled plants failed to set seed); and for G. bulbifera 119 seeds per plant were counted (Table 2). The difference between the species from humid and arid environments becomes obvious if seed production is compared with the average size of replacement bulbs: if bulb volume is estimated as that of a sphere from the figures for diameter (Table 2), G. lutea produces 0.1 seeds/mm3 bulb volume, but numbers for G. graeca and G. bulbifera are 4.8 and 13.7, respectively. Figures for bulbil production per mm3 replacement bulb volume are comparable (0.13 vs. 0.07 and 0.11 bulbils/mm3 bulb volume). Due to the switch for G. lutea the latter figure was calculated from the average dimeter of bulbil-producing, non-flowering plants (5.00 ± 1.51 mm). This indicates a clear shift in reproductive output from bulbils (G. lutea) towards seeds (G. bulbifera, G. graeca).
Gagea lutea differs from the two arid-zone species by another important character: a switch in ontogenesis between bulbil production and seed production (Schnittler et al. 2009). If plants gain resources (indicated by larger replacement bulbs), they produce first only bulbils, not flowers. Above a certain threshold in parent bulb size, only flowers, no bulbils, are developed. In such species, the resource threshold (expressed as diameter of the parent bulb) for bulbil production is always lower than that for flower/seed development (Schnittler et al. 2013). Species lacking a switch start to develop a single to several bulbils as soon as the replacement bulb has reached a certain diameter and then continue to form them indefinitely throughout the life of the plant. In such species, the threshold for flower/seed development may be lower than that for bulbil development, as it is the case in G. bulbifera and G. graeca (Table 2). From this reason, a direct comparison in terms of resources allocated to bulbils vs. seeds is impossible due to the switch in G. lutea: plants of this species never invest simultaneously into seeds and bulbils.
The results of AFLP genotyping reflect the consequences of a) seed and bulbil production and b) absence/presence of a switch for genetic diversity in a species. The AFLP data for G. graeca showed a small proportion of clonal plants (9%, Fig. 7), with only five pairs of clonal plants found in the first of the two transects. This is comparable with results from G. bulbifera (0% clonal plants, Beisenova et al. 2015) but not with the two remaining species of the genus with known data (G. lutea, 62%, Pfeiffer et al. 2011; G. spathacea, 100%, Pfeiffer et al. 2012). Only flowering plants of G. graeca were used for the AFLP analysis, which all can be expected to form one bulbil/year. In fact, in the neighborhood of a plant (the second member of a pair) we found in 50 cases a non-clonal plant (likely to be germinated from seeds, but we cannot rule out the possibility that they originate from the bulbil of a third plant which was not sampled), in five cases a clonal plant (derived from a bulbil of the first plant). If we compare these relationships (bulb to seed production 1:68 = 0.014; clonal to non-clonal descendants 5:50 = 0.100), we obtain roughly a sevenfold higher establishment rate of bulbils compared with seeds. The two structures are different in size: bulbils are nearly spherical and on average 0.48 mm in diam., equaling a volume of 0.46 mm3; seeds are flattened and approximately 0.1 × 0.15 × 1.3 mm, equaling a volume (if estimated as that of an ellipsoid) of 0.08 mm3. These differences should influence the amount of nutrients stored as well as the desiccation tolerance and explain why bulbils are likely to have a higher chance of establishment.
These considerations suggest that it is not the failure of bulbils to recruit new plants, but the comparatively high seed production of Gagea graeca that leads to a high genetic diversity of the populations. Seed production should nevertheless face a limit in arid climates: the more and the earlier in ontogenesis resources will be invested in seeds, the lesser resources remain for the replacement bulb which guarantees survival of the plant. But the relation volume to surface which we must assume to be an important parameter for desiccation tolerance, decreases with decreasing diameter of the bulb. In contrast to G. bulbifera, where bulbs can hold effectively water via sclerophyllous roots (see Levichev 1999), the bulbs of G. graeca lack this adaptation and may thus face a high risk of desiccation during the long Mediterranean summer. This limits the proportion of resources invested into seeds, since plants with large bulbs can be expected to have a higher chance of survival. Indeed, the average size of the bulbils is higher in G. graeca in comparison to G. bulbifera (3 vs. 2.5 mm), and so is the proportion of nitrogen invested into the replacement bulb (62% vs. 36%, Table 2). However, figures for relative investment into plant parts cannot be compared directly from two reasons: first, the measurements for G. lutea were obtained at the peak of flowering season, these for G. bulbifera near seed maturation, whereas the measured plants of G. graeca were already completely dried at the time of measurement. Therefore, storage processes may have been not yet completed in the first two species; which should lead to an underestimation of the relative investment into dormant parts (bulbs, bulbils, seeds) but overestimation for vegetative parts in the first two species. Second, G. lutea shows a switch – bulbils and seeds are never found at one plant, which does not allow a direct comparison.
Growth patterns in Gagea seem to be rather stable for a species (Levichev 2013). With a quantitative analysis of the morphology for a sampled population, thresholds for bulbil formation and flowering can be defined (Schnittler et al. 2009). For all hitherto investigated species from humid climates, thresholds for bulbil formation are lower than these for flower formation (Schnittler et al. 2013); the exception are the two species from arid climates, Gagea bulbifera and G. graeca (Table 2). Such shifts towards earlier generative reproductions may be a general trend for perennial herbs in arid habitats. Franks and Weis (2015) observed in a 5-year drought experiment with Brassica rapa that drought selected for plants that flowered at a smaller size and earlier ontogenetic stage. These changes seem to correspond with a lower level of neoteny and a much smaller genome in the arid-zone species (see discussion in Peruzzi et al. 2009). For Gagea lutea, direct measurements resulted in DNA contents (1C) of 19.75 pg (Greilhuber et al. 2000) and 21.35 pg (Zonneveld et al. 2015). Respective values for the species from arid environments, inferred from chromosome total haploid length are 4.00–6.96 (mean 5.48) pg for the species of sect. Antericoides and 8.65 pg for G. bulbifera (Peruzzi 2012a).
In contrast to the differences mentioned above, the trend towards andromonecy (weak plants produce male flowers only, and even in larger plants the last flower is often male) seems to be a general tendency in the genus. For Gagea lutea, this was reported by Nishikawa (1998), and male flowers were reported as well in the two species from arid zones. In the plants of G. graeca investigated for morphology we counted 95 flowering plants with one flower only, in 57 of these the flower was apparently hermaphroditic (mean bulb diam. 2.51 mm), in 38 it was apparently male (mean bulb diam. 2.31 mm). This is indirectly confirmed by the high proportion of weak sterile plants (30%) in the cohort sampled for seed set. An androdioecious breeding system was reported for several species in the genera Gagea, especially the species formerly assigned to Lloydia (Patterson and Givnish 2002; Manicacci and Despres 2001; Peruzzi et al. 2008). This fits into the pattern of frequent occurrence of female-sterile breeding systems observed in Liliaceae and was suggested to result from size-dependent sex allocation (Peruzzi 2012b). In addition to the finding that often the smallest plants of a cohort are male (Lilium apertum, Zhang et al. 2014; Fritillaria montana, Peruzzi et al. 2012), in Gagea spp. usually the last flowers, when the replacement bulb may run out of resources, are male. We did not investigate the pollination system for G. graeca but assume facultative autogamy, which was found as well in G. lutea (Pfeiffer et al. 2013). For G. graeca, Peruzzi et al. (2008) obtained viable seeds from isolated plants in cultivation.
In accordance with the general habit of the plants, our data suggest that the two species from arid environments (Gagea graeca and G. bulbifera), although belonging to divergent clades in phylogenetic trees, are more similar in life history than to G. lutea from a humid climate (Table 2). Not directly accessible is plant age, where the most remarkable differences may be expected. Since in Gagea the plants replace all structures of the vegetation body completely every year, and only the replacement bulb survives dormancy periods, only a mark-revisit approach over several years would provide reliable age data. In the field we found for G. graeca only a low proportion (28%) of non-flowering plants. Looking for the distribution of plants over size classes of 0.25 bulb diameter, the highest number of plants (56) was found for 2.50–2.75 mm diam. We therefore assume that G. graeca is a short-lived perennial and may flower and produce seeds already in the second year of its life (see also Peruzzi et al. 2008).
Adaptations to arid environments in the genus Gagea (Liliaceae) are characterized by (i) a predominance of sexual reproduction and (ii) a lower reproductive age (lower threshold for flower development). This is connected with a reduction in size (especially bulb diameter). In contrast to most species of the genus, Gagea graeca seems to be a short-lived perennial, possessing several traits that are rather typical for annual plants.
MS designed the study, carried out most of the data analyses and wrote the manuscript, assisted by AP, JP, and AN. Field work and morphological analyses were carried out by AP, JP and MS, data for seed set were contributed by CB. AFLP analyses were performed by AN and AK; the C/N analyses by AN. All authors read and approved the final manuscript.
For generous help with accommodation and logistics we which to thank Andreas Traxler (Lentas, Crete), for help with plant measurements the student participants of the field trip to Crete 2013. Mohamed el Dadamouny, Greifswald, helped with compiling the map of occurrences.
The authors declare that they have no competing interests.
Availability of data and materials
Three datasets supporting the conclusions of this article are available in the Open Science Framework repository https://osf.io/3htcp/files/ in the format Microsoft Excel (doi:10.17605/OSF.IO/3HTCP); explanations are added to the files.
Consent for publication
Not applicable, no data of any persons were used.
Ethics approval and consent to participate
Not applicable, the study involves no human participants.
The project received no special funding. AN wants to thank as well the authorities of L.N. Gumilyev Natl. Univ., Astana, Kazakhstan, for supporting three research stays in Germany.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Ashman TL (1994) Reproductive allocation in hermaphrodite and female plants of Sidalcea oregana ssp. spicata (Malvaceae) using four currencies. Am J Bot 81:433–438View ArticleGoogle Scholar
- Beisenova S, Peterson A, Peterson J, Bersimbayev RI, Klahr A, Schnittler M (2015) On the limits of drought—life history of Gagea bulbifera. Flora 210:72–79View ArticleGoogle Scholar
- Carta A, Peruzzi L (2016) Testing the large genome constraint hypothesis: plant traits, habitat and climate seasonality in Liliaceae. New Phytol 210(2):709–716View ArticlePubMedGoogle Scholar
- Danin A, Danin B (2015) Flora of Israel online. Jerusalem. http://flora.org.il/en/plants/. Accessed 10 Nov 2016
- Douhovnikoff V, Dodd RS (2003) Intra-clonal variation and a similarity threshold for identification of clones: application to Salix exigua using AFLP molecular markers. Theor Appl Genet 106:1307–1315View ArticlePubMedGoogle Scholar
- Franks SJ, Weis AE (2015) A change in climate causes rapid evolution of multiple life-history traits and their interactions in an annual plant. J Evol Biol 21:1321–1334View ArticleGoogle Scholar
- Greilhuber J, Ebert I, Lorenz A, Vyskot B (2000) Origin of facultative heterochromatin in the endosperm of Gagea lutea (Liliaceae). Protoplasma 212:217–226View ArticleGoogle Scholar
- Greuter W (1970) The taxonomic position of Lloydia graeca (Liliaceae) and related species. Israel J Bot 19:155–160Google Scholar
- Jahn R, Schönfelder P, Mayer A, Scheuerer M (1995) Exkursionsflora für Kreta. Ulmer, p 446Google Scholar
- Leitch IJ, Beaulieu JM, Chase MW, Leitch AR, Fay MF (2010) Genome size dynamics and evolution in monocots. J Bot 2010:e862516Google Scholar
- Levichev IG (1999) Zur Morphologie in der Gattung Gagea Salisb. (Liliaceae) I Die unterirdischen Organe. Flora 194:379–392View ArticleGoogle Scholar
- Levichev IG (2013) Structural features of shoots in Lloydia, Gagea, Kharkevichia (Liliaceae) as evolutionary variability of the modules of mesome nature in monokotyledons. Bot Zhurn 98:409–453 (In Russian) Google Scholar
- Manicacci D, Despres L (2001) Male and hermaphrodite flowers in the alpine lily Lloydia serotina. J Bot 79:1107–1114View ArticleGoogle Scholar
- Nishikawa Y (1998) The function of multiple flowers of a spring ephemeral, Gagea lutea (Liliaceae), with reference to blooming order. Canad J Bot 76:1404–1411View ArticleGoogle Scholar
- Patterson TB, Givnish TJ (2002) Phylogeny, concerted convergence, and phylogenetic niche conservatism in the core Liliales: insights from rbcL and ndhF sequence data. Evolution 56:233–252View ArticlePubMedGoogle Scholar
- Peruzzi L (2003) Contribution to the cytotaxonomical knowledge of Gagea Salisb. (Liliaceae) sect. Foliatae A. Terracc. and synthesis of karyological data. Caryologia 56:115–128View ArticleGoogle Scholar
- Peruzzi L (2008) Contribution to the cytotaxonomical knowledge of the genus Gagea Salisb. (Liliaceae). III New karyological data from the central Mediterranean area. Caryologica 61(1):92–106View ArticleGoogle Scholar
- Peruzzi L (2012a) Chromosome diversity and evolution in Gagea (Liliaceae). Bocconea 24:147–158Google Scholar
- Peruzzi L (2012b) Male flowers in Liliaceae are more frequent than previously thought. Bocconea 24:301–304Google Scholar
- Peruzzi L (2016) A new infrafamilial taxonomic setting for Liliaceae, with a key to genera and families. Plant Biosyst 150:1341–1347View ArticleGoogle Scholar
- Peruzzi L, Tison J-M, Peterson A, Peterson J (2008) On the phylogenetic position and taxonomic value of Gagea trinervia (Viv.) Greuter and Gagea sect. Anthericoides A. Terracc. (Liliaceae). Taxon 57(4):1201–1214Google Scholar
- Peruzzi L, Leitch IJ, Caparelli KF (2009) Chromosome diversity and evolution in Liliaceae. Ann Bot 103:459–475View ArticlePubMedGoogle Scholar
- Peruzzi L, Mancuso E, Gargano D (2012) Males are cheaper, or the extreme consequence of size/age dependent sex allocation: sexist gender diphasy in Fritillaria montana (Liliaceae). Bot J Linn Soc 168(3):323–333View ArticleGoogle Scholar
- Peterson A, John H, Koch E, Peterson J (2004) A molecular phylogeny of the genus Gagea (Liliaceae) in Germany inferred from non-coding chloroplast and nuclear DNA sequences. Plant Syst Evol 245:145–162View ArticleGoogle Scholar
- Peterson A, Levichev IG, Peterson J (2008) Systematics of Gagea and Lloydia (Liliaceae) and infrageneric classification of Gagea based on molecular and morphological data. Mol Phyl Evol 46:446–465View ArticleGoogle Scholar
- Peterson A, Levichev IG, Peterson J, Harpke D, Schnittler M (2011) New insights into the phylogeny and taxonomy of Chinese species of Gagea (Liliaceae)—speciation through hybridization. Org Div Evol 11:387–407View ArticleGoogle Scholar
- Peterson A, Harpke D, Levichev IG, Beisenova S, Schnittler M, Peterson J (2016) Morphological and molecular investigations of Gagea (Liliaceae) in southeastern Kazakhstan with special reference to putative altitudinal hybrid zones. Plant Syst Evol 302:985–1007View ArticleGoogle Scholar
- Pfeiffer T, Klahr A, Heinrich A, Schnittler M (2011) Does sex make a difference? Genetic diversity and spatial genetic structure in two co-occurring species of Gagea (Liliaceae) with contrasting reproductive strategies. Plant Syst Evol 292:189–201View ArticleGoogle Scholar
- Pfeiffer T, Klahr A, Peterson A, Levichev IG, Schnittler M (2012) No sex at all? Extremely low genetic diversity in Gagea spathacea (Liliaceae) across Europe. Flora 207:372–378View ArticleGoogle Scholar
- Pfeiffer T, Harter DEV, Formella N, Schnittler M (2013) Reproductive isolation vs. inter-breeding between Gagea lutea (L.) Ker Gawl. and G. pratensis (Pers.) Dumort. (Liliaceae) and their putative hybrids in Mecklenburg–Western Pomerania. Plant Spec Biol 28:193–203View ArticleGoogle Scholar
- Schnittler M, Eusemann P (2010) Consequences of genotyping errors for estimation of clonality: a case study on Populus euphratica Oliv. (Salicaceae). Evol Ecol 24:1417–1432View ArticleGoogle Scholar
- Schnittler M, Pfeiffer T, Harter DEV, Hamann A (2009) Bulbils contra seeds: reproductive investment in two species of Gagea (Liliaceae). Plant Syst Evol 279:29–40View ArticleGoogle Scholar
- Schnittler M, Peterson A, Peterson J, Beisenova S, Bersimbaev RI, Pfeiffer T (2013) Minor differences with big consequences: reproductive patterns in the genus Gagea (Liliaceae). Flora 208:591–598View ArticleGoogle Scholar
- Strid A (2016) Atlas of the aegean flora. Part 2. Maps. Englera 33(2):1–878Google Scholar
- Tekşen M, Erkul SK (2015) The synopsis of the genus Gagea (Liliaceae) in Turkey. Phytotaxa 230:101–129View ArticleGoogle Scholar
- Tison J-M (2016) Gagea graeca. The IUCN red list of threatened species 2016: eT13142235A18611875. http://dx.doi.org/102305. Accessed 10 Nov 2016
- Tison J-M, Peterson A, Harpke D, Peruzzi L (2013) Reticulate evolution of the critical Mediterranean Gagea sect Didymobulbos (Liliaceae) and its taxonomic implications. Plant Syst Evol 299:413–438View ArticleGoogle Scholar
- Veselý P, Bureš P, Šmarda P, Pavlíček T (2012) Genome size and DNA base composition of geophytes: the mirror of phenology and ecology? Ann Bot 109:65–75View ArticlePubMedGoogle Scholar
- Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414View ArticlePubMedPubMed CentralGoogle Scholar
- Zarrei M, Wilkin P, Fay MF, Ingrouille MJ, Zarre S, Chase MW (2009) Molecular systematics of Gagea and Lloydia (Liliaceae; Liliales): implications of analyses of nuclear ribosomal and plastid DNA sequences for infrageneric classification. Ann Bot 104:125–142View ArticlePubMedPubMed CentralGoogle Scholar
- Zarrei M, Wilkin P, Ingrouille MJ, Chase MW (2011) A revised infrageneric classification for Gagea (Tulipeae; Liliaceae): insights from DNA sequence and morphological data. Phytotaxa 15:44–56Google Scholar
- Zhang Z-Q, Zhu X-F, Sun H, Yang Y-P, Barrett SCH (2014) Size dependent gender modification in Lilium apertum (Liliaceae): does this exhibit gender diphasy? Ann Bot 114: 441–453View ArticlePubMedPubMed CentralGoogle Scholar
- Zimowski M, Leuschner HH, Gärtner H, Bergmeier E (2014) Age and diversity of Mediterranean dwarf shrublands: a dendrochronological approach along an altitudinal gradient on Crete. J Veget Sci 25:122–134View ArticleGoogle Scholar
- Zonneveld BJM, te Linde B, van den Berg L-J (2015) Genome sizes of 227 accessions of Gagea (Liliaceae) discriminate between the species from the Netherlands and reveal new ploidies in Gagea. Springerplus 4:395View ArticlePubMedPubMed CentralGoogle Scholar